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In brief, cells were washed twice with phosphate-buffered saline and resuspended in prewarmed (37 °C) Seahorse basal medium (Agilent) supplemented with 1 mM pyruvate, 2 mM glutamine, and 5 mM glucose (pH 7.4)

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Size:1000 mcg/ml - 10 ml (Pack of 3)

To comprehensively show the influence of Ldhb on metabolic pathways, we performed metabolomic profiling of 4T1-Vector and 4T1- Ldhb cells