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After incubation for at least 1 h, the DTT was removed by loading the sample onto the bead bed of a MiniTrap desalting column pre-equilibrated with 100 mM HEPES p H 7.0

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[34] [35] Television [edit] Grillo first appeared on television in episodes of Silk Stalkings (1993), and Poltergeist: The Legacy (1996) before being cast as businessman Hart Jessup on the daytime soap opera Guiding Light in 1996 up until 1999