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To selectively and compatibly modify peptides and proteins, an orthogonal chemical reaction must be (1) selective toward other potential reactive functional groups, (2) proceed with very fast reaction rates, (3) under physiological pH, and (4) at room temperature or 37 °C
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PFK catalyzes the rate-limiting phosphorylation of fructose-6-phosphate to fructose-1,6-diphosphate
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