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Next, the Fc-tag was captured with CaptivA − HF protein-A affinity resin and the PreSission protease with Glutathione Sepharose 4C (Cytiva)

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SufR coordinates two [4Fe-4S] 2+,1+ clusters and functions as a transcriptional repressor of the sufBCDS operon and an autoregulator of sufR in cyanobacteria