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The aromatic side chain of Y31 of IGF1 is sandwiched between P5 in the L1 domain and P256 in the CRD, and is further surrounded by several other conserved hydrophobic residues in the CRD, including F241, F251, and I255 (Fig
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Therefore, we studied cell cultures under ultralow attachment conditions in which cancer cells form 3D tumor colonies or spheroids and mimic soft agar experiments, but allow easier experimental manipulation
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